Coding

Part:BBa_I741106:Design

Designed by: Garrett Tobin   Group: iGEM07_Penn_State   (2007-06-24)


xylA and xylB metabolizing proteins plus region inbetween


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1587
    Illegal PstI site found at 1959
    Illegal PstI site found at 2338
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1587
    Illegal PstI site found at 1959
    Illegal PstI site found at 2338
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1409
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1587
    Illegal PstI site found at 1959
    Illegal PstI site found at 2338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1587
    Illegal PstI site found at 1959
    Illegal PstI site found at 2338
    Illegal NgoMIV site found at 1614
    Illegal AgeI site found at 997
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 115


Design Notes

xylA and xylB are together in this part because it is useful to have both proteins expressed at once, rather than making 2 parts that must be combined for functionality.


Source

E. coli K-12 genome: reverse compliment of 3725940 to 3728788

References